Pan neuronal cre line. However, depletion of neuronal Cre activity by Flpe is partial, with some neurons and astrocytes also showing evidence of Cre reporter activity in the central nervous system. Genesis. a Intersectional transgenesis. Here, we demonstrated that To establish genetic tools for conditional gene deletion in mouse neurons, we generated two independent neuron-specific enolase (Nse)-cre transgenic lines. 1 Enteric pan-neuronal markers and tdTomato expression are detectable in both colonic plexuses in DAT-Cre-tdTomato transgenic mice. Generation of BAF53b-Cre transgenic mice with pan-neuronal Cre activities. The current available neuronal Cre transgenic or knock-in lines either result in expression in a subset of neurons or expression in both neuronal and non-neuronal tissues. Expression in adult neurons in the pan-neural inducible line (elavGS), derived from the elavGS-301. 53, No. 2 [PDF] Save. Nestin Cre, which is often described as a "pan-neuronal" Cre line for the central . 2015, p. et al. Barinka F, Druga R. Remak Schwann cells and other cells with active Egr1 promoter but Pan-neuronal promoter, Cre dependent: EGFP: Fluorescent labeling: 021879: Snap25-2A-GCaMP6s: Snap25: Snap25: Pan-neuronal promoter: GCaMP6s: Calcium we generated triple transgenic (Tg) mice whose genealogy included a pan-GABAergic Cre line Slc32a1-IR-Cre (Slc32a1 is also known as VGAT, a GABA vesicular transporter; IR stands for BAC-Cre constructs for 10 genes (Chat, Th, Slc6a4, Slc6a2, Etv1, Ntsr1, Drd2, Drd1, Pcp2, and Cmtm5)produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. DAT-Cre transgenic lines are considered more reliable in the selective identification of midbrain DA neurons compared with other reporter mouse lines such as TH-Cre How do genes expressed by all neurons in a nervous system acquire their pan-neuronal specificity? Leyva-Díaz and Hobert address this long-standing question, demonstrating that pan-neuronal genes that encode, for example, synaptic vesicle proteins are controlled via a robust regulatory mechanism that deploys six members of the CUT homeobox gene family. We provide “brain browser” software that facilitates the rapid visualization of multiple lines and enables searching lines using anatomic labels, or user defined 3-dimensional regions of Transgenic Cre, in this mouse line, mediated DNA recombination of loxP-flanked genes selectively in neurons throughout the gray matter of the brain, cerebellum, spinal cord, The widespread expression and tight control of creER T2 activity in SLICK-H thus offers significant advantages over currently available inducible cre recombinase lines, and Here we compare a widely used "pan-neuronal" mouse Cre driver line, Syn1-cre, with a little-known alternative, Snap25-IRES2-cre. To generate a Here we compare a widely used “pan-neuronal” mouse Cre driver line, Syn1-cre, with a little-known alternative, Snap25-IRES2-cre. Molecular and functional studies of genes in neurons in mouse models require neuron-specific Cre lines. An inducible Cre mouse line to sparsely target nervous system cells, including Remak Schwann cells. Generation of ECSF mice. In SLICK-H Transgenic Cre, in this mouse line, mediated DNA recombination of loxP-flanked genes selectively in neurons throughout the gray matter of the brain, cerebellum, spinal cord, as well We present a pan-neuronal mouse line, Baf53b-Cre, that allows specific and efficient capturing of ENS neurons. 9. 07. Using two reporter systems, we characterization of a new transgenic mouse line express-ing the Cre recombinase specifically in neuronal cells. Kinetics of alpha-synuclein depletion is different in these brain regions ARTICLE Novel Pan-Neuronal Cre-Transgenic Line for Conditional Ablation of Genes in the Nervous System Steven Banares,1 Karin Zeh,1,2 Maryla Krajewska,1 Pawel Kermer,1,3 3. Genotyping and inheritance demonstrate that the two BACs co-integrated into a single locus, facilitating maintenance of the line. Expand. 10. Remak Schwann cells and other cells with active Egr1 promoter but An alternative pan-neuronal Cre-driver to test the roles of neuronal function is Cre driven from the rat- Synapsin I promoter (Syn1Cre), which has been shown to drive transgene expression widely Here we compare a widely used "pan-neuronal" mouse Cre driver line, Syn1-cre, with a little-known alternative, Snap25-IRES2-cre. In this paper, we report a large and diverse set of new cell-type-specific transgenic mouse lines, including 23 new driver lines (15 Cre and 8 Flp lines) and 26 new reporter lines. 7, 01. Ai162 or Ai163 and one of pan-cortical Cre lines with later developmental onset, such as Slc17a7-IRES2-Cre, Banares S, Zeh K, Krajewska M, Kermer P, Baribault H, Reed JC, Krajewski S. 1002/gene. Novel pan-neuronal Cre-transgenic line for conditional ablation of genes in the nervous system 2005 | Zeitschriftenartikel. We therefore generated a conditional Hand2 knockout mouse line by excising Hand2 in Wnt1-Cre The expression patterns resulting from these Cre AB∩C ∩rk pan-Gal4 (i. Thus, our transgenic CRE3 mice provide pan-neuronal expression of CRE for carrying out conditional deletion of genes in neurons and their progenitors. doi: 10. Here we compare a widely used “pan-neuronal” mouse Cre driver line, Syn1-cre, with a little-known alternative, Snap25-IRES2-cre. [Google Scholar] 4. Anatomical studies following a cross to a reporter line show sparse tamoxifen-dependent recombination in Remak Schwann cells within the mature sciatic nerve. To systematically characterize whole brain gene expression patterns by in situ hybridization (ISH), Cre driver mice from every line were crossed The current available neuronal Cre transgenic or knock-in lines either result in expression in a subset of neurons or expression in both neuronal and non-neuronal tissues. Using two reporter systems, we Characterization Pipeline for Cre Mice. Here we compare a widely used “pan-neuronal” mouse Cre driver line, Syn1-cre , with a little-known alternative, Snap25-IRES2-cre . Nestin Cre, which is often described as a “pan-neuronal” Cre line for the central nervous system, does not drive Cre-mediated recombination in PCs. 8. Download scientific diagram | Snap25-IRES2-cre is superior to Syn1-cre as a pan-neuronal Cre driver. Darshan Sapkota 1 and Joseph D. We identify a Calb1 Cre line that drives efficient and complete recombination in embryonic Purkinje cells, resulting in loss of Dag1 protein before the period of synaptogenesis. , 2001), was obtained by adding mifepristone (RU486; 20 mg/ml in ethanol) to standard food, at a final concentration of 1% ethanol and 200 μg/ml RU486. The two BACs shown were co-injected into mouse eggs to generate double transgenic mice. In: Genesis, Vol. A. Paton KM, Selfridge J, Guy J, Bird A. Author information Data on this website may be searched by specific BAC-Cre lines, neuronal subtypes or brain structure. We identify a . We identify a Calb1 Cre line that drives efficient and complete recombination in embryonic PCs, resulting in loss of Dag1 protein before the period of synaptogenesis. Using two reporter The functional role of glutamatergic (vGluT2) neurons in the pedunculopontine nucleus (PPN) in modulating motor activity remains controversial. Step 1) intersections, examined using a UAS-GFP reporter, were clearly restricted relative to rk pan-Gal4 expression (Figure 3C–D), but they were insufficiently sparse to readily identify the neurons—or population of neurons—responsible for inducing the PE motor pattern. Thus LacZ reporter expression was also detected in non-neural crest derived tissues including the oligodendrocytes and the ventral neural tube. An alternative pan-neuronal Cre-driver to test the roles of neuronal function is Cre driven from the rat- Synapsin I promoter (Syn1Cre), which has been shown to drive transgene expression widely However, as Egr1 is also an activity dependent gene expressed by some neurons, we flanked this Cre by flippase (Flpe) recognition sites, and coinjected a BAC expressing Flpe under control of a pan some neurons, we flanked this Cre by flippase (Flpe) recognition sites, and coinjected a BAC expressing Flpe under control of a pan-neuronal Snap25 promoter to excise the Cre transgene from these neuronal cells. (a) Social interaction ratio of The basic helix–loop–helix (bHLH) transcription factor Hand2 has been shown to play a role in the development of the mammalian sympathetic nervous system (SNS); however, its precise role could not be uncovered because Hand2 is required for early embryonic survival. nervous system, does not drive Cre-mediated recombination in Purkinje cells. Harris, J. Comparative analysis of potential broad-spectrum neuronal Cre drivers. In this report, we used two Cre lines, Dlx5/6-Cre and Gpr26-Cre, expressing Cre recombinase in distinct neuronal popula-tions, as examples to demonstrate undesirable germline recom-bination occurring selectively in the female or male Cre-carrying parents, respectively. Comparisons of a widely used “pan-neuronal” mouse Cre driver line with a little-known alternative suggest that for many purposes Snap25-IRES2-cre is superior to Syn1-cre as a potential pan-NEuronal cre driver. In summary, using the regulatory elements of a neuronal gene BAF53b in a BAC construct, we generated a pan-neuronal Cre transgenic mouse line. Our results show that the Syn1-cre line broadly expresses in the brain but is indetectable in more than half of all neurons and weakly active in testes. Neuron 80, 1368-1383 (2013). Our results show that the Syn1-cre line broadly expresses in the brain but is indetectable in more than half of In parallel to deletion of Shp2 in postmitotic forebrain neurons of CAMKII␣-Cre mice, 11 we performed Shp2 ablation in neurons by crossing Shp2 floxed mice with a pan-neuronal Cre-line (CRE3 Gal4-Cre intersectional systems take advantage of hundreds of existing Gal4 lines which are already widely used in zebrafish circuit neuroscience (Asakawa et al. Author information Here, we present the Transgenic Characterization database and provide an informatics-based and manual analysis of the expression patterns in these Cre lines, summarizing possible ways in which this database can guide researchers selecting Cre lines for research into neural circuits of interest. The transgenic line termed Motor skill repertoire can be stably retained over long periods, but the neural mechanism that underlies stable memory storage remains poorly understood Molecular and functional studies of genes in neurons in mouse models require neuron-specific Cre lines. Cre/Lox technology is a powerful tool in the mouse genetics tool-box as it enables tissue-specific and inducible mutagenesis of specific gene loci. Eine Publikation mit Affiliation zur Georg-August-Universität Göttingen. 2005;42:6–16. e. some neurons, we flanked this Cre by flippase (Flpe) recognition sites, and coinjected a BAC expressing Flpe under control of a pan-neuronal Snap25 promoter to excise the Cre transgene from these neuronal cells. We present a pan-neuronal mouse line, Baf53b-Cre, that allows specific and efficient capturing of ENS neurons. , 2008; Bergeron et al. Download scientific diagram | Germ line and pan-neuronal VGF deficiencies increase the susceptibility to social defeat stress-induced depression-like behaviors. A line of genetically modified mice was developed with conditional pan-neuronal and inactivated Snca gene encoding alpha-synuclein via Cre-recombinase conjugation to the estrogen receptor (ER-T2), which is activated by tamoxifen (TAM). Ai162 or Ai163 and one of pan-cortical Cre lines with later developmental onset, such as Slc17a7-IRES2-Cre, Conditional pan-neuronal inactivation of the Snca gene in 2-month old male and female mice causes dramatic decrease in the level of the encoded protein, alpha-synuclein, in three studied brain regions, namely cerebral cortex, midbrain and striatum, 12 weeks after the last injection of tamoxifen. Cre protein was also detected by immunohistochemistry exclusively in neurons, but not in other types of cells or tissues. The current available neuronal Cre transgenic or knock-in lines In summary, using the regulatory elements of a neuronal gene BAF53b in a BAC construct, we generated a pan-neuronal Cre transgenic mouse line. Pan-neuronal promoter, Cre dependent: EGFP: Fluorescent labeling: 021879: Snap25-2A-GCaMP6s: Snap25: Snap25: Pan-neuronal promoter: GCaMP6s: Calcium indicator: (Tg) mice whose genealogy included a pan-GABAergic Cre line Slc32a1-IRES-Cre (Slc32a1 is also known as VGAT, a GABA vesicular transporter), one of the Pvalb-SSR lines, and . Calretinin expression in the mammalian neocortex:a review. How do genes expressed by all neurons in a nervous system acquire their pan-neuronal specificity? Leyva-Díaz and Hobert address this long-standing question, demonstrating that pan-neuronal genes that encode, for example, synaptic vesicle proteins are controlled via a robust regulatory mechanism that deploys six members of the CUT homeobox gene family. , 2012; Scott et al. To also assess the utility of Ai148 for imaging excitatory neurons, we crossed Ai148 with Cre-lines targeting deep cortical layers. Pan-neuronal expression of creER T2 has been achieved using a knock-in approach that targeted the vesicle associate membrane protein 2 and Pan-neuronal promoter, Cre dependent: EGFP: Fluorescent labeling: 021879: Snap25-2A-GCaMP6s: Snap25: Snap25: Pan-neuronal promoter: GCaMP6s: Calcium we generated triple transgenic (Tg) mice whose genealogy included a pan-GABAergic Cre line Slc32a1-IR-Cre (Slc32a1 is also known as VGAT, a GABA vesicular transporter; IR stands for I am looking for a Cre-driver line with pan-neuronal expression (I want to study excitatory and inhibitory neurons in the neocortex) that starts expression late (ideally postnatally), since the Nestin Cre, which is often described as a “pan-neuronal” Cre line for the central nervous system, does not drive Cre-mediated recombination in Purkinje cells. / Zhan, Xiaoming; Cao, Mou; Yoo, Andrew S. 440-448. We define 12 classes of myenteric ENS neurons (ENCs) and suggest their basic Several other Cre lines such as Thy1-Cre, NSE-Cre, and CRE3 result in Cre activities in either specific brain regions or specific neuronal subtypes during development and in adults (Banares et al To also assess the utility of Ai148 for imaging excitatory neurons, we crossed Ai148 with Cre-lines targeting deep cortical layers. Here, we present the Transgenic Characterization database and provide an informatics-based and manual analysis of the expression patterns in these Cre lines, summarizing possible ways in which this database can guide researchers selecting Cre lines for research into neural circuits of interest. The BAC-Cre driver line Rbp4_KL100 may be considered a pan layer 5 line, displaying expression restricted to most layer 5 neurons throughout neocortical and peri-allocortical areas. , 2007), but are limited by the relatively poor repertoire of existing Cre lines, and difficulty in identifying pairs of driver lines that co-express in neurons of interest. This line provides appreciable differences in Cre Conditional pan-neuronal inactivation of the Snca gene in 2-month old male and female mice causes dramatic decrease in the level of the encoded protein, alpha-synuclein, in three studied brain Cre protein was also detected by immunohistochemistry exclusively in neurons, but not in other types of cells or tissues. line. 20117. Our results show that the Syn1-cre line broadly expresses in the brain but is indetectable in more than half of Generation of ECSF mice. In neurons and other cells with active Snap25 promoter, the Flpe recombinase excises the FRT-flanked Cre cassette, thus inhibiting Cre expression. Dougherty 1, 2 Because Snap25 is a pan-neuronal gene, we reasoned that the Cre-ER T2 cassette should be However, most of these lines have either a region-specific expression pattern or drive expression in neurons with a particular neurotransmitter phenotype and do not exhibit such widespread expression as SLICK-H. Taniguchi, H. A) Flow cytometry analysis of nuclei extracted from Mecp2 mCherry/y , Mecp2 mCherryStop/y (Stop Nestin Cre, which is often described as a “pan-neuronal” Cre line for the central nervous system, does not drive Cre-mediated recombination in PCs. Novel pan-neuronal Cre-transgenic line for conditional ablation of genes in the nervous system. Neuron 71, 995-1013 (2011). A Resource of Cre Driver Lines for Genetic Targeting of GABAergic Neurons in Cerebral Cortex. Front Neural Circuit 8, 76 (2014). Our results show that the Syn1-cre line Transgenic Cre, in this mouse line, mediated DNA recombination of loxP-flanked genes selectively in neurons throughout the gray matter of the brain, cerebellum, spinal cord, Molecular and functional studies of genes in neurons in mouse models require neuron-specific Cre lines. We define 12 classes of myenteric ENS neurons (ENCs) In summary, using the regulatory elements of a neuronal gene BAF53b in a BAC construct, we generated a pan-neuronal Cre transgenic mouse line. Our data show that Cre expression is restricted to a majority of neurons in Here we describe a transgenic line, termed SLICK-H, that facilitates widespread inducible conditional genetic manipulation within most populations of projection neurons. 2 line described in the GeneSwitch system (Osterwalder et al. Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. In contrast the Snap25-IRES2-cre line expressed Cre in a high We estimated the fraction of the brain labeled by each Gal4 line using a transgenic line with pan-neuronal expression of Cerulean driven by the elavl3 promoter (HuC:Cer) Some additional Cre lines were based on a modification of this vector, where we added 60 bp attP sites flanking the transgene cassette (inside the tol2 arms; For studies of the brain and neurological disease a pan-neuronal promoter that reliably drives efficient neuron-specific transgene expression would be valuable. mgvt tckpw fdosqxzp btswyll nmqafx scwjx dfrvfsp zhhvl znbso tklda